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Fungal DNA was extracted from 43 samples of impacted low-melt AGAR by a 3-step extraction method and amplified by QPCR.
真菌dna从43份压缩空气采用三步提取法和QPCR法进行扩增来提取的。
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As the QPCR technique continues to develop and improve, it will be more and more wildly applied in the field of environmental microorganisms.
随着该技术的进一步完善，其必将在环境微生物领域发挥越来越重要的作用。
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Objective To set up a duplex quantitative real-time PCR (QPCR) assay with high sensitivity, specificity and rapidity to detect Candida krusei and Candida glabrata.
目的建立一种快速、灵敏、特异的鉴定克柔念珠菌和光滑念珠菌的双重实时荧光定量PCR方法。
1. 实时荧光定量PCR （Quantitative Real-time PCR）是一种在DNA扩增反应中，以荧光化学物质测每次聚合酶链式反应（PCR）循环后产物总量的方法。通过内参或者外参法对待测样品中的特定DNA序列进行定量分析的方法。· Real-timePCR是在PCR扩增过程中，通过荧光信号，对PCR进程进行实时检测。由于在PCR扩增的指数时期，模板的Ct值和该模板的起始拷贝数存在线性关系，所以成为定量的依据。