Using reverse transcription PCR, kininogen mRNA was also detected in lamprey gut, kidney, and leukocyte, but absent in lamprey buccal gland.

运用反转录PCR技术，检测出激肽原还存在于七鳃鳗的肠、肾和白细胞中，但不存在于口腔腺中。

Methods Using reverse transcription-polymerase chain reaction(RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.

方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。

Methods We detected HFRSV RNA in urinary sediments of 79 samples from 31 HFRS patients by nested reverse transcription polymerase chain reaction (nested RT PCR).

方法应用套式-逆转录-聚合酶链反应对31例肾综合征出血热患者79份尿液沉渣细胞中病毒核酸进行检测。

Methods 40 patients with lung cancer were studied to detect TTF-1 gene by using reverse transcription-polymerase chain reaction assay(RT-PCR).

方法采用逆转录—聚合酶链反应（RT-PCR）方法检测40例患者的肺癌组织TTF-1基因的表达。

Methods Reverse transcription-PCR (RT-PCR) was used to analyze the expression of CASP9 in 81 NSCLC and normal lung tissues.

方法应用逆转录-PCR方法检测了81例NSCLC患者及对应癌旁正常组织中CASP9的表达情况；