Screening and sequencing present 120 valid sequences, 84 of them belong in expressed sequence tags (ESTs).

通过克隆鉴定和序列测定，获得120个有效片段，其中84个认定为序列表达标签（EST）。

The technology of expressed sequence tags (ESTs) was broadly applied in analysis of gene function and expression pattern.

表达序列标签）测序分析技术广泛应用于基因功能和表达模式的分析研究。

These ESTs were classified into 11 different functional categories, the highest proportion of which was related to protein synthesis.

同源序列功能分类大致分为11类，与蛋白质合成有关的蛋白所占比例最大。

The majority of these ESTs were co-expressed in response to desiccation and osmotic stress and were generally not induced in response to heat and cold stress.

这些EST中的大多数在对干旱和渗透压胁迫发生反应时是共表达的，而在耐受冷热胁迫时并不被诱导表达。

Conclusion It is a feasible method to discover and acquire novel genes of S. japonicum by ESTs, combination of primer-walking sequncing and bioinformatics technique.

结论表达序列标签法、步移法测序和生物信息学技术有利于发现日本血吸虫新基因。