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Initial composting, the number of DGGE bands was 20.
表现为堆肥初期,DGGE条带数较多,为20。
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After eight months, we detected the communities of bacterias and archaeas using the PCR-DGGE technique.
培养八个月之后,采用PCR - DGGE技术对烃降解培养物进行菌群解析。
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The PCR-DGGE profiles showed that the preponderant populations were steady during butyrate-type fermentation period.
根据PCR DGGE指纹图谱显示此时期丁酸型发酵优势菌群在反应器内结构保持稳定。
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It was suggested that the denaturant concentration range and electrophoresis time may be the key factors for DGGE experiment.
提示合理的变性剂浓度范围和电泳时间是DGGE实验的关键因素。
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These results suggest that DGGE followed by clone technique is a practicable protocol to research the complex community of soil microbe.
这表明DGGE和克隆技术相结合的方法是研究土壤微生物群落结构的一种可行方法。
1. DGGE(denaturing gradient gel electrophoresis),即变性梯度凝胶电泳,是根据DNA在不同浓度的变性剂中解链行为的不同而导致电泳迁移率发生变化,从而将片段大小相同而碱基组成不同的DNA片段分开。